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MSU Faculty Authors

Author

Dongyou Liu

Assistant Research Professor, CVM Basic Science

E-mail address: liu@cvm.msstate.edu
Website: http://www.cvm.msstate.edu/basic_sciences/faculty/liu_dongyou.html


Education

  • D.V.M. - Hunan Agricultural University
  • Ph.D. - Melbourne University


Special Interests
Molecular microbiology
Parasite immunology

Primary Area of Expertise
Molecular and immunological identification of microbial pathogens; bacterial pathogenesis; Immunological control of microbial and other diseases of medical and veterinary importance


Manual of Security Sensitive Microbes and Toxins

Liu, Dongyou
Publisher: CRC Press
2014
ISBN: 9781466553965

Security sensitive microbes (viruses, bacteria, fungi, and parasites) and toxins, which are often referred to as select agents and toxins, have the capacity to cause serious illness and death in humans, animals, and plants. Throughout history, these microbes and toxins have been exploited in one form or another as biowarfare and bioterror agents that create fear and panic well beyond any actual physical damages they might cause.

Manual of Security Sensitive Microbes and Toxins provides comprehensive, state-of-the-art coverage of microbes and toxins of biosecurity concern. The ultimate goal is to increase our awareness of these agents and enhance our preparedness against any future bio-emergencies.

The book begins with an introduction containing a brief overview of the historical aspects of security sensitive microbes and toxins. This is followed by a concise summary of the current status in relation to the regulation of security sensitive microbes and toxins and a discussion of future development trends.

The book is divided into seven parts:
• Microbes and Toxins Affecting Humans and Animals: Viruses
• Microbes and Toxins Affecting Humans and Animals: Bacteria
• Microbes and Toxins Affecting Humans and Animals: Fungus and Parasite
• Microbes and Toxins Affecting Humans and Animals: Toxins
• Microbes Affecting Animals: Viruses
• Microbes Affecting Animals: Bacteria
• Microbes Affecting Plants

Written by experts in the relevant areas of research, the chapters are authoritative reviews, each one covering a single microbe or toxin with respect to its classification, biology, epidemiology, pathogenesis, identification, diagnosis, treatment, and prevention. The chapters also discuss the limitations of our current knowledge and challenges relating to improved detection and control of the microbe or toxin.



Molecular Detection of Human Parasitic Pathogens

Liu, Dongyou
Publisher: CRC Press
2012
ISBN: 9781439812426

A book on the molecular detection of major human viral, bacterial, fungal, and parasitic pathogens, this text provides coverage of the organisms with an emphasis on the sample preparation and molecular detection procedures. Chapters consist of concise reviews of pathogens in respect to taxonomy, biology, epidemiology, and pathogenesis; a description of a clinical sample collection preparation procedures; a summary of molecular detection methods available; and a discussion on the challenges, limitations, and advantages for current methods. Written for practitioners and students, this textbook is a guide for future development of improved molecular methods for pathogens. Offers an in-depth review of state-of-the-art molecular detection and identification methods for human parasitic pathogens. Provides comprehensive coverage of important human parasitic pathogens. Provides an authoritative summary of biology, epidemiology, and pathogenesis of major human parasitic pathogens. Includes chapter entries written by experts in respective fields of human parasitic pathogen collection.



Molecular Detection of Human Bacterial Pathogens

Liu, Dongyou
Publisher: CRC Press
2011
ISBN: 9781439812389

As more original molecular protocols and subsequent modifications are described in the literature, it has become difficult for those not directly involved in the development of these protocols to know which are most appropriate to adopt for accurate identification of bacterial pathogens. Molecular Detection of Human Bacterial Pathogens addresses this issue, with international scientists in respective bacterial pathogen research and diagnosis providing expert summaries on current diagnostic approaches for major human bacterial pathogens.

Each chapter consists of a brief review on the classification, epidemiology, clinical features, and diagnosis of an important pathogenic bacterial genus, an outline of clinical sample collection and preparation procedures, a selection of representative stepwise molecular protocols, and a discussion on further research requirements relating to improved diagnosis.

This book represents a reliable and convenient reference on molecular detection and identification of major human bacterial pathogens; an indispensable tool for upcoming and experienced medical, veterinary, and industrial laboratory scientists engaged in bacterial characterization; and an essential textbook for undergraduate and graduate students in microbiology.



Molecular Detection of Human Fungal Pathogens

Liu, Dongyou
Publisher: CRC Press
2011
ISBN: 9781439812402

The large number of molecular protocols available creates a dilemma for those attempting to adopt the most appropriate for streamlined identification and detection of fungal pathogens of interest. Molecular Detection of Human Fungal Pathogens provides a reliable and comprehensive resource relating the molecular detection and identification of major human fungal pathogens.

This volume contains expert contributions from international mycologists involved in fungal pathogen research and diagnosis. Following a similar format throughout, each chapter comprises:

  • A brief review of the classification, epidemiology, clinical features, and diagnosis of one or a group of related fungal species
  • An outline of clinical sample collection and preparation procedures
  • A selection of representative stepwise molecular detection protocols
  • A discussion on further research requirements for improving the diagnosis


  • The book offers an indispensable tool for medical, veterinary, and industrial laboratory scientists working in the area of fungal determination. It also constitutes a convenient textbook for undergraduate and graduate students majoring in microbiology and is an essential guide for upcoming and experienced laboratory scientists wishing to acquire and polish their skills in molecular diagnosis of fungal diseases.



    Molecular Detection of Human Viral Pathogens

    Liu, Dongyou
    Publisher: CRC Press
    2010
    ISBN: 9781439812365

    The first in a series of four books, this comprehensive work details the molecular detection of major human viral pathogens. Focusing on sample preparation and molecular detection procedures, each chapter presents a concise review of the pathogen concerned including its taxonomy, biology, epidemiology and pathogenesis; a description of clinical sample collection preparation procedures; a summary of molecular detection methods; representative molecular detection protocols; and a discussion on the challenges, limitations, and advantages for the current methods as well as further research required to improve diagnosis.



    Handbook of Nucleic Acid Purification

    Liu, Dongyou
    Publisher: CRC Press
    2009
    ISBN: 1420070967

    An all-encompassing volume on nucleic acid purification strategies for bacteria, viruses, fungi, parasites, mammals, and plants, this book emphasizes sample preparation methods for direct molecular applications. Each chapter provides a concise review of historical background and purification principles, a detailed description of current techniques, including several state-or-art protocols for large and small scale preparations, and a discussion for future development trends. A comprehensive reliable reference, the book supplies a useful roadmap for clinical, forensic, and research scientists involved in molecular analysis of biological specimens.



    Molecular Detection of Foodborne Pathogens

    Liu, Dongyou
    Publisher: CRC Press
    2009
    ISBN: 1420076434

    While the vast majority of our food supuplies are nutritious and safe, foodborne pathogen-related illness still affects millions of people each year. Large outbreaks of foodborne diseases - such as the recent salmonella outbreak linked to various peanut butter products - continue to be reported with alarming frequency.

    One of the most effective ways to control and prevent human foodborne infections is to implement a pathogen surveillance system. Addressing the biology, epidemiology, and pathogenesis of more than 60 microorganisms, Molecular Detection of Foodborne Pathogens demonstrates how to implement a surveillance system that uses state-of-the-art molecular techniques to quickly detect and identify bacterial, fungal, viral, and parasitic pathogens concerned.

    Included in each chapter:

  • Concise review of the selected pathogen in respect to its biology, epidemiology, and pathogenesis
  • Summary of molecular detection methods available
  • Description of clinical/food sample collection and preparation procedures
  • Selection of robust, effective, step-by-step detection protocols
  • Discussion of the current challenges and ongoing research needs to further extend utility and performance of molecular disgnostic methods
  • With proven, ready-to-use protocols (including commercial kits), this globally pertinent resource demonstrates how speedy and effective detection methods can lead to more lives saved and a consistently safe, quality, and trustworthy food supply.



    Handbook of Listeria monocytogenes

    Liu, Dongyou
    Publisher: CRC Press
    2008
    ISBN: 1420051407
    ISSN: 978-1420051407

    Once feared as a deadly intracellular bacterium with the extraordinary capacity to survive a wide array of arduous external stressors, Listeria monocytogenes is increasingly recognized as a preferred vector for delivering anti-infective and anti-cancer vaccine molecules.

    A reliable, single-source reference on the fundamental aspects of this bacterium is crucial to support future study and further the advancement of biomedical sciences and intervention strategies.

    Drawn from an international panel of scientists with notable expertise in their respective fields, the Handbook of Listeria monocytogenes is divided into four sections:

    Section I discusses the biology and pathogenicity of this bacterium, including epidemiology and stress responses.

    Section II demonstrates identification and detection techniques such as phenotypic and genotypic identification, strain typing, and virulence determination.

    Section III details the current knowledge of genetic manipulation of Listeria, including comparative genomics, genomic divisions, epidemic clones and population structure, and analysis of cell envelope proteins.

    Section IV covers innate and adaptive immunity against Listeria, and examines the use of this bacterium for anti-infective and anti-cancer vaccine development.

    The first comprehensive compilation of knowledge in this area, this handbook is an indispensable reference for anyone embarking on the path of manipulation of Listeria as either a model for the study of the host-bacterium relationship or as a tool for delivering protective molecules to cytoplasm.




    Liu, Dongyou. "Preparation of Listeria Monocytogenes Specimens for Molecular Detection and Identification" International Journal of Food Microbiology 122.3 (2008): 229-242.
    Liu, Dongyou, Mark L. Lawrence, and A. Jerald Ainsworth. "A Novel PCR Assay for Listeria Welshimeri Targeting Transcriptional Regulator Gene LWE1801" Journal of Rapid Methods and Automation in Microbiology 16.2 (2008): 154-163.
    Liu, Dongyou, Mark L. Lawrence, and Anthony D. Hitchins. "Molecular Characterization of Listeria Monocytogenes Strains Harboring Listeria Innocua Putative Transcriptional Regulator Gene LIN0464" Journal of Rapid Methods and Automation in Microbiology 16.4 (2008): 412-427.
    Liu, Dongyou , Mark L. Lawrence, Frank W. Austin, and A. Jerald Ainsworth . "A multiplex PCR for species- and virulence-specific determination of Listeria monocytogenes" Journal of Microbiological Methods 71.2 (2007): 133-140.
    Liu, Dongyou , Mark L. Lawrence, Lesya M. Pinchuk, A. Jerald Ainsworth, and Frank W. Austin. "Characteristics of cell-mediated, anti-listerial immunity induced by a naturally avirulent Listeria monocytogenes serotype 4a strain HCC23." Archives of Microbiology 188.3 (2007): 251-256.
    Liu, Dongyou , Mark L. Lawrence, A. Jerald Ainsworth, and Frank W. Austin. "Toward an improved laboratory definition of Listeria monocytogenes virulence" International Journal of Food Microbiology 118.2 (2007): 101-115.
    Liu, Dongyou. "Listeria-Based Anti-Infective Vaccine Strategies" Recent Patents on Anti-Infective Drug Discovery 1.3 (2006): 281-290.
    Liu, Dongyou. "Identification, Subtyping and Virulence Determination of Listeria monocytogenes, An Important Foodborne Pathogen" Journal of Medical Microbiology 55.6 (2006): 645-659.
    Liu, Dongyou , Mark L. Lawrence, Lisa Gorski, Robert E. Mandrell, A. Jerald Ainsworth, and Frank W. Austin. "Listeria monocytogenes Serotype 4b Strains Belonging to Lineages I and III Possess Distinct Molecular Features" Journal of Clinical Microbiology 44.1 (2006): 214-217.
    Liu, Dongyou , Mark L. Lawrence, Frank W. Austin, A. Jerald Ainsworth, and Lanny W. Pace. "PCR detection of pathogenic Leptospira genomospecies targeting putative transcriptional regulator genes" Canadian Journal of Microbiology 52.3 (2006): 272-277.
    Liu, Dongyou , Edwin J. Swiatlo, Frank W. Austin, and Mark L. Lawrence. "Use of a putative transcriptional regulator gene as target for specific identification of Staphylococcus epidermidis" Letters in Applied Microbiology 43.3 (2006): 325-330.
    Liu, Dongyou , Mark L. Lawrence, Martin Wiedmann, Lisa Gorski, Robert E. Mandrell, A. Jerald Ainsworth, and Frank W. Austin. "Listeria monocytogenes Subgroups IIIA, IIIB, and IIIC Delineate Genetically Distinct Populations with Varied Pathogenic Potential" Journal of Clinical Microbiology 44.11 (2006): 4229-4233.
    Liu, Dongyou , Mark L. Lawrence, A. Jerald Ainsworth, and Frank W. Austin. "Comparative assessment of acid, alkali and salt tolerance in Listeria monocytogenes virulent and avirulent strains" FEMS Microbiology Letters 243.2 (2005): 373-378.
    Liu, Dongyou , Chinling Wang, Edwin J. Swiatlo, and Mark L. Lawrence. "PCR amplification of a species-specific putative transcriptional regulator gene reveals the identity of Enterococcus faecalis" Research in Microbiology 156.9 (2005): 944-948.
    Liu, Dongyou , Mark L. Lawrence and Frank W. Austin. "Evaluation of PCR primers from putative transcriptional regulator genes for identification of Staphylococcus aureus" Letters in Applied Microbiology 40.1 (2005): 69-73.
    Liu, Dongyou , Mark L. Lawrence, A. Jerald Ainsworth, and Frank W. Austin. "Isolation and PCR Amplification of a Species-Specific Oxidoreductase-Coding Gene Region in Listeria grayi" Canadian Journal of Microbiology 51.1 (2005): 95-98.
    Liu, Dongyou , Susan Hollingshead, Edwin Swiatlo, Mark L. Lawrence, and Frank W. Austin. "Rapid identification of Streptococcus pyogenes with PCR primers from a putative transcriptional regulator gene" Research in Microbiology 156.4 (2005): 564-567.
    Liu, Dongyou , Mark L. Lawrence, A. Jerald Ainsworth, amd Frank W. Austin. "Species-specific PCR determination of Listeria seeligeri" Research in Microbiology 155.9 (2004): 741-746.
    Liu, Dongyou , A. Jerald Ainsworth, Frank W. Austin, and Mark L. Lawrence. "Use of PCR primers derived from a putative transcriptional regulator gene for species-specific determination of Listeria monocytogenes" International Journal of Food Microbiology 91.3 (2004): 297-304.
    Liu, Dongyou , A. Jerald Ainsworth, Frank W. Austin, Mark L. Lawrence. "Identification of a gene encoding a putative phosphotransferase system enzyme IIBC in Listeria welshimeri and its application for diagnostic PCR" Letters in Applied Microbiology 38.2 (2004): 151-157.
    Liu, Dongyou , A. Jerald Ainsworth, Frank W. Austin, and Mark L. Lawrence. "PCR detection of a putative N-acetylmuramidase gene from Listeria ivanovii facilitates its rapid identification" Veterinary Microbiology 101.2 (2004): 83-89.
    Liu, Dongyou. "Listeria monocytogenes: comparative interpretation of mouse virulence assay" FEMS Microbiology Letters 233.1 (2004): 159-164.
    Liu, Dongyou , Mark L. Lawrence, and Frank W. Austin. "Specific PCR identification of Pasteurella multocida based on putative transcriptional regulator genes" Journal of Microbiological Methods 58.2 (2004): 263-267.
    Liu, Dongyou , A. Jerald Ainsworth, Frank W. Austin, and Mark L. Lawrence. "Characterization of virulent and avirulent Listeria monocytogenes strains by PCR amplification of putative transcriptional regulator and internalin genes" Journal of Medical Microbiology 52.12 (2003): 1065-1070.
    Liu, Dongyou , A. Jerald Ainsworth, Frank W. Austin, and Mark L. Lawrence. "Identification of Listeria innocua by PCR targeting a putative transcriptional regulator gene" FEMS Microbiology Letters 223.2 (2003): 205-210.